中文摘要：

植物的先天免疫限制了對(duì)全球食品安全構(gòu)成威脅的細(xì)菌病原體的生長(zhǎng)。然而，植物免疫抑制細(xì)菌生長(zhǎng)的機(jī)制仍然不清楚。在這里，我們展示了擬南芥分泌的天冬氨酸蛋白酶1和2（SAP1和SAP2）切割進(jìn)化上保守的細(xì)菌蛋白MucD，以重復(fù)地抑制細(xì)菌病原體銅綠假單胞菌的生長(zhǎng)。SAP1的抗菌活性在植物內(nèi)和體外都需要其蛋白酶活性。過表達(dá)SAP1的植物表現(xiàn)出增強(qiáng)的MucD切割和抗性，但在生長(zhǎng)和繁殖上沒有受到影響，而sap1 sap2雙突變植物表現(xiàn)出MucD切割和對(duì)P. syringae的抗性受損。缺乏mucD的P. syringae在植物內(nèi)和體外的生長(zhǎng)受損。值得注意的是，在植物內(nèi)和體外，缺乏可切割的MucDF106Y的ΔmucD的生長(zhǎng)不受SAP活性的影響。我們的研究發(fā)現(xiàn)了植物抗菌機(jī)制背后的遺傳因素和生化過程。

英文摘要：

Plant innate immunity restricts growth of bacterial pathogens that threaten global food security. However, the mechanisms by which plant immunity suppresses bacterial growth remain enigmatic. Here we show that Arabidopsis thaliana secreted aspartic protease 1 and 2 (SAP1 and SAP2) cleave the evolutionarily conserved bacterial protein MucD to redundantly inhibit the growth of the bacterial pathogen Pseudomonas syringae. Antibacterial activity of SAP1 requires its protease activity in planta and in vitro. Plants overexpressing SAP1 exhibit enhanced MucD cleavage and resistance but incur no penalties in growth and reproduction, while sap1 sap2 double mutant plants exhibit compromised MucD cleavage and resistance against P. syringae. P. syringae lacking mucD shows compromised growth in planta and in vitro. Notably, growth of ΔmucD complemented with the non-cleavable MucDF106Y is not affected by SAP activity in planta and in vitro. Our findings identify the genetic factors and biochemical process underlying an antibacterial mechanism in plants.

論文信息：

論文題目：Site-specific cleavage of bacterial MucD by secreted proteases mediates antibacterial resistance in Arabidopsis

期刊名稱：Nature Communications

時(shí)間期卷：10, Article number: 2853 (2019)

在線時(shí)間：2019年6月28日

DOI：doi.org/10.1038/s41467-019-10793-x

產(chǎn)品信息：

貨號(hào)：101-A

規(guī)格：100ug

品牌：Virogen

產(chǎn)地：美國(guó)

名稱：Anti-Glutathione antibody

授權(quán)代理商：Target Technology（靶點(diǎn)科技）

檢測(cè)方式：WB

谷胱甘肽（glutathione，r-glutamyl cysteingl +glycine，GSH）是一種含γ-酰胺鍵和巰基的三肽，由谷氨酸、半胱氨酸及甘氨酸組成，存在于身體的幾乎每一個(gè)細(xì)胞。谷胱甘肽有還原型（G-SH）和氧化型（G-S-S-G）兩種形式，在生理?xiàng)l件下以還原型谷胱甘肽占絕大多數(shù)。谷胱甘肽還原酶可以催化兩型間的互變，該酶的輔酶還可以為磷酸戊糖旁路代謝提供的NADPH 。Virogen的抗谷胱甘肽抗體Anti-Glutathione antibody貨號(hào)101-A見刊于Nature Communications：

Liposoma巨噬細(xì)胞清除劑Clodronate Liposomes氯膦酸二鈉脂質(zhì)體的材料和方法：

Virogen抗谷胱甘肽抗體Anti-Glutathione antibody貨號(hào)101-A專業(yè)論文WB數(shù)據(jù)參考